Journal: Advanced Healthcare Materials
Article Title: Clinically Relevant and Precisely Printable Live Adipose Tissue–Based Bio‐Ink for Volumetric Soft Tissue Reconstruction
doi: 10.1002/adhm.202402680
Figure Lengend Snippet: Characterization of ATmf bio‐inks. A) Sequential steps outlining the preparation of the ATmf bio‐ink. B–E) Mechanical analysis of the ATmf bio‐ink based on the ATmf concentration (%v/v): shear sweep analysis (B), dynamic frequency sweep (C), loss tangent (tan 𝛿), and (D), and compressive modulus (E) (n = 5). F,G) Results of 2D printability tests of the ATmf bio‐ink: printable minimum line width (F) and pore fidelity (G) (n = 5) measured after printing 2D line patterns and rectangular pores, respectively. H) Thickness measurements of multilayered structures printed with ATmf bio‐inks (n = 5). Multilayered structures are printed with varying ATmf contents to analyze 3D printability, and their thicknesses are measured. Error bars indicate standard deviations. (*p < 0.05; **p < 0.01; ***p < 0.001).
Article Snippet: To prepare the ATmf bio‐ink, a fibrinogen/gelatin mixture was prepared through the following steps: 3 mg mL− 1 of hyaluronic acid (935166, Millipore Sigma, MA, USA) was dissolved in serum‐free α‐minimum essential medium (MEM, 15‐010‐CV, Corning, NY, USA) overnight by gentle rotation at 37 °C in an oven.
Techniques: Concentration Assay, Shear